Course Content and Learning Goals
Contents
Metabolic engineering
- Microbial cell factory development through metabolic engineering.
- The use of computational modelling and omics data in metabolic engineering.
Computational modelling of metabolism
- Principles of constraint-based modelling, including flux balance analysis and model reconstruction.
- Get hands-on experience in performing simulations with a genome-scale model using the RAVEN Toolbox.
- Proteome- and enzyme-constrained modelling of metabolism.
- Get hands-on experience in simulating enzyme-constrained models with GECKO Toolbox.
- Visualization of flux and other omics data on metabolic pathway maps.
- Constraint-based simulation of microbial communities.
Fermentation technologies
- The various different modes by which microbial bioreactor cultivations can be done.
- Suitability of the different cultivation modes for use with microbial cell factories.
- Learn how to calculate rates from bioreactor cultivations, to use as input for constraint-based models.
Transcriptomics and proteomics analysis
- Learn about the principles of transcriptomics and proteomics for differential gene expression analysis.
- What to consider when designing an experiment.
- How to process the data to ensure high quality analysis.
- Get hands-on experience in converting raw RNAseq data into differential gene expression results.
Integrative data analysis
- How various types of data can be combined to extract new hypotheses from your data.
- Get hands-on experience in performing gene-set enrichment analysis with RNAseq data.
- Learn how proteomics data can be used to constrain enzyme-constrained models.